The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 29, 2019
Filed:
Sep. 24, 2015
The Regents of the University of California, Oakland, CA (US);
Valorbec S.e.c., Montreal, CA;
Vincent J. J. Martin, Montreal, CA;
Lauren Narcross, Montreal, CA;
John E. Dueber, San Francisco, CA (US);
William C. DeLoache, Oakland, CA (US);
Zachary N. Russ, Berkeley, CA (US);
P. James Scrivens, St. Constant, CA;
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US);
VALORBEC S.E.C., Montreal, CA;
Abstract
The present disclosure provides a variant tyrosine hydroxylase that provides for increased production of L-DOPA in a host cell that expresses the tyrosine hydroxylase. The present disclosure provides nucleic acids encoding the variant tyrosine hydroxylase, and host cells genetically modified with the nucleic acids. The present disclosure provides methods of making L-DOPA in a host cell. The present disclosure provides methods of making a benzylisoquinoline alkaloid (BIA), or a BIA precursor. The present disclosure provides methods of detecting L-DOPA level in a cell. The present disclosure provides methods of identifying tyrosine hydroxylase variants that provide for increased L-DOPA production; and methods of identifying gene products that provide for increased tyrosine production.