The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 29, 2019
Filed:
Mar. 11, 2016
Biotech Pharmaceutical Co. Ltd, Beijing, CN;
Centro DE Inmunologia Molecular, Havana, CU;
MeyLen Chea, Havana, CU;
Julio Palacios, Havana, CU;
Miguel Arias, Havana, CU;
Loany Calvo, Havana, CU;
Tamara González, Havana, CU;
Rolando Pérez, Havana, CU;
Zhi Bai, Shanxi Province, CN;
Yuemao Liu, Hunan Province, CN;
Kaiheng Xiao, Beijing, CN;
Xiao Chen, Henan Province, CN;
Zhenhua He, Beijing, CN;
Yangliu Cai, Beijing, CN;
Zhenhua Yang, Shan Xi Sheng, CN;
Xianhong Bai, Beijing, CN;
BIOTECH PHARMACEUTICAL CO. LTD., Beijing, CN;
CENTRO DE INMUNOLOGIA MOLECULAR, Havana, CU;
Abstract
Provided is a method for obtaining high-yield, stable expression cell clones from myeloma cell lines in a protein-free culture medium. The method is used for industrial production of a recombinant antibody, and includes three stage: (1) adapting to a protein-free culture medium, statically culturing cells at a low density, and gradually reducing a fat-rich supplement to a chemical culture medium; (2) adapting to a protein-free culture medium; culturing cells at a high density, and using a perfusion fermentation system in a laboratory scale; and (3) screening high-yield, stable expression cell clones from the cells after fermentation ends. The cell clone may be used to produce a humanized anti-NeuGcGM3 14F7 recombinant antibody.