The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 29, 2019

Filed:

Aug. 03, 2017
Applicants:

Bgi Shenzhen, Shenzhen, Guangdong, CN;

Bgi Shenzhen Co., Limited, Shenzhen, Guangdong, CN;

Inventors:

Ou Wang, Shenzhen, CN;

Xiaofang Cheng, Shenzhen, CN;

Liangying Zou, Shenzhen, CN;

Cankun Chang, Shenzhen, CN;

Hui Jiang, Shenzhen, CN;

Wenwei Zhang, Shenzhen, CN;

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
B01J 19/00 (2006.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
B01J 19/0046 (2013.01); C12N 15/1068 (2013.01); B01J 2219/00317 (2013.01); B01J 2219/00587 (2013.01); B01J 2219/00716 (2013.01); B01J 2219/00722 (2013.01);
Abstract

Provided is a method of constructing a sequencing library. The method includes 1) providing a single-stranded DNA fragment from a biological sample; 2) subjecting the single-stranded DNA fragment to whole genomic amplification to obtain a whole genome amplification product; 3) fragmenting the whole genome amplification product using a transposase embedded with two adaptors to obtain a fragmented product with two adaptors respectively at two ends; and 4) amplifying the fragmented product with two adaptors respectively at two ends using a tag sequence and a pair of primers to obtain said sequencing library.


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