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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10450604 B1

PDFFull Text
Date of Patent:
Oct. 22, 2019

Filed:

Sep. 03, 2015

Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other

Applicant:

Applied Biosystems, Llc, Carlsbad, CA (US);

Inventors:

Willy Wiyatno, Union City, CA (US);

Linda Lee, Palo Alto, CA (US);

David Cox, Foster City, CA (US);

James Nurse, Westport, WA (US);

Douglas Greiner, Fremont, CA (US);

Dennis Lehto, Santa Clara, CA (US);

Janusz Wojtowicz, Sunnyvale, CA (US);

Sam Woo, Redwood City, CA (US);

Richard Reel, Hayward, CA (US);

Assignee:

Applied Biosystems, LLC, Foster City, CA (US);

Attorney:

Jones Robb, PLLC

Primary Examiner:

Brian R Gordon

Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6869 (2018.01); B01L 3/00 (2006.01); F15C 5/00 (2006.01); F16K 99/00 (2006.01); G01N 35/08 (2006.01); G01N 1/14 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6874 (2018.01); G01N 27/447 (2006.01); B01L 3/02 (2006.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); B01L 3/502715 (2013.01); B01L 3/502784 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6874 (2013.01); F15C 5/00 (2013.01); F16K 99/0001 (2013.01); F16K 99/0011 (2013.01); F16K 99/0013 (2013.01); G01N 1/14 (2013.01); G01N 27/44743 (2013.01); G01N 27/44769 (2013.01); G01N 35/08 (2013.01); B01J 2219/0036 (2013.01); B01J 2219/0061 (2013.01); B01J 2219/00353 (2013.01); B01J 2219/00364 (2013.01); B01J 2219/00608 (2013.01); B01J 2219/00612 (2013.01); B01J 2219/00619 (2013.01); B01J 2219/00626 (2013.01); B01J 2219/00637 (2013.01); B01J 2219/00653 (2013.01); B01J 2219/00657 (2013.01); B01J 2219/00659 (2013.01); B01L 3/0293 (2013.01); B01L 7/52 (2013.01); B01L 2200/0673 (2013.01); B01L 2200/10 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/0421 (2013.01); B01L 2400/0487 (2013.01); C12Q 2535/101 (2013.01); F16K 2099/0084 (2013.01); Y10T 137/0318 (2015.04); Y10T 137/4259 (2015.04); Y10T 137/85978 (2015.04); Y10T 137/85986 (2015.04); Y10T 137/86863 (2015.04); Y10T 436/2575 (2015.01);
Abstract

A method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The discrete volumes are separated from one another by a liquid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.

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