The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 22, 2019

Filed:

Sep. 01, 2014
Applicants:

Toyo Kohan Co., Ltd., Chiyoda-ku, Tokyo, JP;

Yamaguchi University, Yamaguchi-shi, Yamaguchi, JP;

Inventors:

Shuichi Kamei, Kudamatsu, JP;

Mayuko Hosoya, Kudamatsu, JP;

Masaaki Oka, Ube, JP;

Shoichi Hazama, Ube, JP;

Ryouichi Tsunedomi, Ube, JP;

Assignees:

Toyo Kohan Co., Ltd., Tokyo, JP;

Yamaguchi University, Yamaguchi-shi, Yamaguchi, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C07H 21/00 (2006.01); C12Q 1/6837 (2018.01); C12N 15/09 (2006.01); C12Q 1/6816 (2018.01); C12Q 1/6832 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6837 (2013.01); C12N 15/09 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6832 (2013.01);
Abstract

A buffer composition for hybridization of a target nucleic acid is provided. The nucleic acid can include a nucleotide to be detected with a nucleic acid probe. The probe can contain a nucleotide sequence complementary to the target nucleic acid. The buffer can include a blocking nucleic acid having a nucleotide sequence complementary to a non-target nucleic acid having a nucleotide not to be detected corresponding to the nucleotide to be detected. The buffer composition can suppress non-specific hybridization to the nucleic acid probe even when a non-target nucleic acid is present. The use of the buffer composition can achieve excellent detection efficiency of the target nucleic acid.


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