The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 22, 2019

Filed:

May. 19, 2015
Applicant:

Agriculture Victoria Services Pty Ltd, Atwood, Victoria, AU;

Inventors:

Noel Oliver Ian Cogan, Macleod, AU;

Hiroshi Shinozuka, Kingsbury, AU;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C40B 50/06 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6806 (2018.01);
U.S. Cl.
CPC ...
C12N 15/10 (2013.01); C12N 15/1093 (2013.01); C40B 50/06 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6869 (2013.01);
Abstract

A method of preparing an at least partially randomly sheared library of nucleic acids is provided. The method includes the steps of providing a source of nucleic acids, randomly incorporating modified bases into the nucleic acids, and digesting the modified nucleic acids with one or more modification-dependent restriction endonucleases to produce the nucleic acid library. The practice of the method can be facilitated using a kit for performing the method. The method can be used to form nucleic acid libraries and as part of a method of next generation sequencing.


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