The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 15, 2019

Filed:

Mar. 22, 2018
Applicant:

The Board of Trustees of the University of Illinois, Urbana, IL (US);

Inventors:

Stephen A. Boppart, Champaign, IL (US);

Haohua Tu, Savoy, IL (US);

Sixian You, Urbana, IL (US);

Yuan Liu, Dublin, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 21/00 (2006.01); G06T 7/00 (2017.01); G01N 33/483 (2006.01); G01N 21/65 (2006.01); G01N 21/64 (2006.01); G16B 40/00 (2019.01); G01N 21/63 (2006.01); G01N 15/14 (2006.01); G01N 15/10 (2006.01);
U.S. Cl.
CPC ...
G06T 7/0014 (2013.01); G01N 15/1456 (2013.01); G01N 21/636 (2013.01); G01N 21/6486 (2013.01); G01N 21/65 (2013.01); G01N 33/483 (2013.01); G16B 40/00 (2019.02); G01N 2015/1006 (2013.01); G01N 2015/144 (2013.01); G01N 2021/653 (2013.01); G01N 2201/06113 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/20081 (2013.01); G06T 2207/30024 (2013.01); G06T 2207/30096 (2013.01);
Abstract

Methods for label-free characterization of untagged molecules within a biological sample in-situ. The untagged molecules may be constituent of extracellular vesicles, and are excited in the biological sample with at least one wavelength band of light derived from a single stream of optical pulses. Light emitted by the untagged molecules by SHG, THG, 2PAF and 3PAF processes is detected. Separate measures of the biological sample corresponding to light emitted by the untagged molecules in each of the SHG, THG, 2PAF and 3PAF processes are derived. On that basis, normal extracellular vesicles may be differentiated from extracellular vesicles associated with a tumor on the basis of a specified signature of characteristics of images of SHG, THG, 2PAF and 3PAF processes.


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