The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 15, 2019
Filed:
Jan. 30, 2017
General Electric Company, Schenectady, NY (US);
Ryan Charles Heller, Guilderland, NY (US);
John Richard Nelson, Clifton Park, NY (US);
Paresh Lakhubhai Patel, Cardiff, GB;
Alison Myfanwy Wakefield, Cardiff, GB;
Stephen James Capper, Cardiff, GB;
Peter James Tatnell, Cardiff, GB;
Jeffrey Kenneth Horton, Vale of Glamorgan, GB;
General Electric Company, Schenectady, NY (US);
Abstract
Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3' end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (T) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.