The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 15, 2019

Filed:

Nov. 21, 2014
Applicant:

Sena Research, Inc., Marietta, GA (US);

Inventor:

Zhen Huang, Marietta, GA (US);

Assignee:

SeNA Research, Inc., Marietta, GA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 38/46 (2006.01); C07K 1/30 (2006.01); G01N 33/53 (2006.01); C12N 9/22 (2006.01); C12N 9/96 (2006.01); A61K 47/61 (2017.01); G16H 50/50 (2018.01); G06F 19/00 (2018.01);
U.S. Cl.
CPC ...
A61K 38/465 (2013.01); A61K 47/61 (2017.08); C07K 1/30 (2013.01); C12N 9/22 (2013.01); C12N 9/96 (2013.01); C12Y 301/26004 (2013.01); G01N 33/5308 (2013.01); G06F 19/00 (2013.01); G16H 50/50 (2018.01); Y02A 90/26 (2018.01);
Abstract

Methods for crystallizing a molecule of interest, such as a polypeptide, in complex with nucleic acid, including contacting the molecule of interest with selenium-derivatized nucleic acid and crystallizing the molecule of interest/selenium-derivatized nucleic acid complex are provided. Methods for determining the X-ray crystal structure of molecule of interest/selenium-derivatized nucleic acid complexes are also provided. Typically, the method of X-ray crystal structural determination includes selenium single-wavelength anomalous phasing of the selenium-derivatized nucleic acid. In some embodiments the phases for the X-ray crystal structure of the molecule of interest are not provided from another crystal. Also disclosed are methods of affecting a biological process by administering a functional nucleic acid to a cell or a subject and/or by bringing into contact a nuclease and a functional nucleic acid, where the functional nucleic acid is selenium-derivatized nucleic acid.


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