The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 15, 2019
Filed:
Aug. 18, 2014
National Cerebral and Cardiovascular Center, Suita-shi, JP;
Foundation for Biomedical Research and Innovation AT Kobe, Kobe-shi, JP;
Hyogo College of Medicine, Nishinomiya-shi, JP;
National University Corporation Hokkaido University, Sapporo-shi, JP;
Kaneka Corporation, Osaka-shi, JP;
Kenichi Yamahara, Suita, JP;
Akihiko Taguchi, Kobe, JP;
Toshihiro Soma, Nishinomiya, JP;
Shunsuke Ohnishi, Sapporo, JP;
Akira Kobayashi, Takasago, JP;
NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER, Suita-shi, JP;
FOUNDATION FOR BIOMEDICAL RESEARCH AND INNOVATION AT KOBE, Kobe-shi, JP;
HYOGO COLLEGE OF MEDICINE, Nishinomiya-shi, JP;
NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY, Sapporo-shi, JP;
KANEKA CORPORATION, Osaka-shi, JP;
Abstract
An object of the present invention is to provide a method for producing a mesenchymal stromal cell composition, comprising conveniently and aseptically separating high-purity amnion-derived MSCs by performing enzyme treatment only once. According to the present invention, the following are provided: a method for producing a mesenchymal stromal cell composition, comprising: performing enzyme treatment of an amnion with collagenase and thermolysin and/or dispase; and filtering the enzyme-treated amnion through a mesh; a method for producing a cryopreserved mesenchymal stromal cell composition; and a therapeutic agent comprising as an active ingredient the mesenchymal stromal cell composition for a disease selected from graft-versus-host disease, inflammatory bowel disease, systemic lupus erythematosus, liver cirrhosis, or radiation enteritis.