The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 08, 2019

Filed:

Dec. 23, 2015
Applicants:

Institut Pasteur, Paris, FR;

Fondation Imagine, Paris, FR;

Assistance Publique—hopitaux DE Paris, Paris, FR;

Universite Paris Descartes, Paris, FR;

Institut National DE LA Sante ET DE LA Recherche Medicale (In-serm), Paris, FR;

Inventors:

Eberl Gerard, Paris, FR;

Bikard David, Paris, FR;

Schnupf Pamela, Paris, FR;

Cerf Bensussan Nadine, Paris, FR;

Gaboriau-Routhiau Valerie, Clamart, FR;

Sansonetti Philippe, Paris, FR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/20 (2006.01); C12N 15/74 (2006.01); C12N 13/00 (2006.01);
U.S. Cl.
CPC ...
C12N 1/20 (2013.01); C12N 13/00 (2013.01); C12N 15/74 (2013.01); C12N 2502/23 (2013.01);
Abstract

The present invention relates to an in vitro method of culturing a segmented filamentous bacterium strain, comprising co-culturing said segmented filamentous bacterium strain with a eukaryotic host cell, wherein the culture is performed at an Olevel inferior to 5% in a rich tissue culture liquid medium containing bacterial medium components including iron. The present invention also relates to methods for genetically modifying a segmented filamentous bacterium strain comprising a step a culturing the strain in vitro.


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