The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 01, 2019

Filed:

Jan. 17, 2015
Applicant:

National Centre for Cell Science, Pune, Maharashtra, IN;

Inventors:

Sharmila A. Bapat, Maharashtra, IN;

Rutika R. Naik, Maharashtra, IN;

Assignee:

NATIONAL CENTRE FOR CELL SCIENCE, Pune, Maharashtra, IN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6886 (2018.01); G01N 33/574 (2006.01); G01N 33/50 (2006.01); G01N 15/14 (2006.01);
U.S. Cl.
CPC ...
G01N 33/57496 (2013.01); C12Q 1/6886 (2013.01); G01N 33/5011 (2013.01); G01N 33/57484 (2013.01); G01N 33/57492 (2013.01); C12Q 2600/156 (2013.01); G01N 15/1459 (2013.01);
Abstract

The present invention provides a method for concurrent resolution of the cancer stem cell (CSC) derived hierarchy, genetic instability, differentially cycling cells and host cells recruited for performing tumor growth supporting functions; and (ii) quantification, monitoring and analysis of these populations. The first level of analysis can be carried out using either CSC- and progenitor-specific markers or a marker-free approach based on label-chase to resolve the tumor regenerative hierarchy. The next level involves combinatorial quantification of differential DNA-RNA contents to identify recruited host and tumor cell variants resulted from genetic instability and differential cycling within the tumor.


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