The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 01, 2019
Filed:
Oct. 17, 2016
Applicant:
Illumina Cambridge Limited, Nr Saffron Walden, GB;
Inventors:
Roberto Rigatti, Arco, IT;
Jonathan Mark Boutell, Nr Saffron Walden, GB;
Min-Jui Richard Shen, Rancho Santa Fe, CA (US);
Assignee:
ILLUMINA CAMBRIDGE LIMITED, Cambridge, GB;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/686 (2018.01); C09K 3/00 (2006.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C09K 3/00 (2013.01); C12Q 1/686 (2013.01);
Abstract
Methods for amplifying nucleic acids are provided. The methods can be used to minimize sequence specific bias caused by the preferential amplification of certain nucleic acid sequences. Methods are described which can lower the efficiency of AT rich templates relative to GC rich templates, thereby minimizing GC bias during amplification reactions with multiple templates of different sequence. The methods are suited to solid phase amplification, for example, utilizing flow cells.