The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 01, 2019
Filed:
Oct. 15, 2015
Applicant:
Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US);
Inventors:
Junko Kuno, Holmes, NY (US);
Wojtek Auerbach, Ridgewood, NJ (US);
David M. Valenzuela, Yorktown Heights, NY (US);
Assignee:
Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/074 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0696 (2013.01); C12N 2500/32 (2013.01); C12N 2500/38 (2013.01); C12N 2500/44 (2013.01); C12N 2500/60 (2013.01); C12N 2501/235 (2013.01); C12N 2501/727 (2013.01); C12N 2509/00 (2013.01);
Abstract
Methods and compositions are provided for generating or maintaining human iPS cells in culture. Methods include the use of a low osmolality medium to make human iPS cells, or use of a low osmolality medium to maintain human iPS cells. Methods for making targeted genetic modification to human iPS cells cultured in low osmolality medium are also included. Compositions include human iPS cells cultured and maintained using the low osmolality medium defined herein.