The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 24, 2019

Filed:

Nov. 12, 2014
Applicant:

Katholieke Universiteit Leuven, Leuven, BE;

Inventors:

Jeroen Lammertyn, Neerijse, BE;

Karel Knez, Aalst, BE;

Filip Delport, Temse, BE;

Assignee:

FOX BIOSYSTEMS NV, Temse, BE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6825 (2018.01); C12Q 1/6851 (2018.01); C12Q 1/6862 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6825 (2013.01); C12Q 1/6851 (2013.01); C12Q 1/6862 (2013.01);
Abstract

A method and kits are provided for nucleic acid quantification and discrimination using surface plasmon resonance (SPR). The method provided is able to significantly enhance the detection limit and multiplex the discrimination assay using the melting properties of the target DNA on top of standard PCR reaction. By using the heating and cooling cycles of the polymerase chain reaction (PCR) or Ligation chain reaction (LCR), DNA is melted and hybridized onto the SPR sensor surface together with a nanoparticle label. Thus, during every cycle of DNA amplification, the quantity and type of target DNA can be monitored.


Find Patent Forward Citations

Lammertyn, Jeroen. (2019). Monitoring dna amplification (U.S. Patent No. 10421990). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/10421990

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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