The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 17, 2019
Filed:
May. 21, 2014
Uniwersytet Jagielloński, Kraków, PL;
Tomasz Gosiewski, Kraków, PL;
Monika Brzychczy-Wloch, Kraköw, PL;
Agata Pietrzyk, Kraków, PL;
Malgorzta Bulanda, Kraków, PL;
Uniwersytet Jagiellonski, Kraków, PL;
Abstract
An exemplary embodiment describes a method for detection of bacteria and fungi in a sample of biological material, wherein the DNA contained in the sample of biological material is subjected to amplification in multiplex real-time PCR, with the use of primers specific for bacteria in the first stage and primers specific for fungi, and in the second stage, the resulting DNA is amplified using primers and probes differentiating fungi into a group of mold fungi and yeast fungi and bacteria into Gram-positive and Gram-negative bacteria. Another exemplary embodiment refers to oligonucleotide primers for the detection of bacteria and fungi by PCR and a kit for simultaneous detection of bacteria and fungi.