The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 17, 2019

Filed:

Jan. 27, 2017
Applicant:

Gen-probe Incorporated, San Diego, CA (US);

Inventors:

Steven T. Brentano, Santee, CA (US);

Dmitry Lyakhov, San Diego, CA (US);

James D. Carlson, San Diego, CA (US);

Norman C. Nelson, San Diego, CA (US);

Lyle J. Arnold, Poway, CA (US);

Michael M. Becker, San Diego, CA (US);

Assignee:

GEN-PROBE INCORPORATED, San Diego, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6881 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6834 (2018.01); C12Q 1/6865 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6881 (2013.01); C12Q 1/6834 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/6865 (2013.01); C12Q 2525/155 (2013.01); C12Q 2525/186 (2013.01); C12Q 2525/197 (2013.01); C12Q 2531/143 (2013.01); C12Q 2565/543 (2013.01);
Abstract

Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.


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