The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 17, 2019

Filed:

Jun. 04, 2018
Applicants:

Steven a Benner, Gainesville, FL (US);

Zunyi Yang, Gainesville, FL (US);

Inventors:

Steven A Benner, Gainesville, FL (US);

Zunyi Yang, Gainesville, FL (US);

Assignee:

Other;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6869 (2018.01); C12P 19/34 (2006.01); C12Q 1/6844 (2018.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); C07H 21/04 (2013.01); C12P 19/34 (2013.01); C12Q 1/6844 (2013.01); C12Q 2521/101 (2013.01); C12Q 2525/117 (2013.01);
Abstract

Disclosed are processes that use DNA polymerases to extend primers annealed to templates, wherein a standard nucleotide in the template guiding the extension directs the incorporation of a nonstandard nucleotide analog into the product duplex opposite that standard template nucleotide, and processes wherein a nonstandard nucleotide in the template guiding the extension directs the incorporation of a standard nucleotide analog opposite the nonstandard template nucleotide. Conditions, including the pH of the mixture where the primer extension is done and the composition of triphosphate mixtures where the primary extensions done, are disclosed.


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