The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 10, 2019
Filed:
May. 09, 2016
Applicant:
The Regents of the University of California, Oakland, CA (US);
Inventors:
Todd Prentice Coleman, La Jolla, CA (US);
Phillip Kyriakakis, La Jolla, CA (US);
Assignee:
The Regents of the University of Califonia, Oakland, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/04 (2006.01); C07H 21/02 (2006.01); C12M 1/34 (2006.01); C12Q 1/6844 (2018.01); C12M 1/00 (2006.01); C07H 1/00 (2006.01); C07H 21/00 (2006.01);
U.S. Cl.
CPC ...
C07H 21/04 (2013.01); C07H 1/00 (2013.01); C07H 21/00 (2013.01); C07H 21/02 (2013.01); C12M 1/00 (2013.01); C12M 1/34 (2013.01); C12Q 1/6844 (2013.01); C12Q 2533/107 (2013.01); C12Q 2565/543 (2013.01);
Abstract
Disclosed herein are methods of assembling nucleic acid fragments, such as non overlapping nucleic acid fragments, in an orientation-specific, sequence-independent way. The methods entail annealing one or more nucleic acid fragments to be assembled onto a solid phase via matching barcodes having sequences complementary to the solid phase oligos, ligating the nucleic acid fragments by end-to-end ligation, and recovering the ligated nucleic acid from the solid phase.