The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 27, 2019

Filed:

May. 31, 2017
Applicant:

T. Hasegawa Co., Ltd., Tokyo, JP;

Inventors:

Kuniki Kino, Tokyo, JP;

Toshiki Furuya, Chiba, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/02 (2006.01); C07C 13/52 (2006.01); C07C 49/215 (2006.01); C12N 15/66 (2006.01); C12P 7/26 (2006.01);
U.S. Cl.
CPC ...
C12Y 117/99 (2013.01); C07C 13/52 (2013.01); C07C 49/215 (2013.01); C12N 15/66 (2013.01); C12P 7/26 (2013.01); C12Y 111/02004 (2013.01); C12Y 114/14 (2013.01); C07C 2602/30 (2017.05);
Abstract

The present invention provides a method of producing (−)-rotundone from α-guaiene. The method includes the steps of: (1) allowing a cytochrome P450 protein to act on α-guaiene, which cytochrome P450 belongs to the CYP152 family and is capable of oxidizing the methylene group at position 3 of α-guaiene to the carbonyl group; and/or (2) allowing a cytochrome P450 protein to act on α-guaiene in the presence of an electron transfer protein capable of transferring electrons to the cytochrome P450 protein, which cytochrome P450 belongs to the CYP152, CYP106, or CYP107 family and is capable of oxidizing the methylene group at position 3 of α-guaiene to the carbonyl group.


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