The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 27, 2019

Filed:

Nov. 20, 2015
Applicants:

Richard Goodman, Lake Oswego, OR (US);

Michael Cohen, Portland, OR (US);

Lulu Cambronne, West Linn, OR (US);

Melissa Stewart, Oregon City, OR (US);

Inventors:

Richard Goodman, Lake Oswego, OR (US);

Michael Cohen, Portland, OR (US);

Lulu Cambronne, West Linn, OR (US);

Melissa Stewart, Oregon City, OR (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/25 (2006.01); C12N 9/00 (2006.01); C12Q 1/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/25 (2013.01); C12N 9/93 (2013.01); C12Q 1/008 (2013.01); C12Y 605/01002 (2013.01); G01N 2333/9015 (2013.01);
Abstract

A polypeptide biosensor that detects free NADis disclosed. The polypeptide comprises a first fragment from an NADdependent DNA ligase acetylation domain, a second fragment from the NADdependent DNA ligase acetylation domain, and a fluorescent protein, wherein the fluorescent protein is positioned between the two DNA ligase acetylation domain fragments. Also disclosed are expression vectors comprising the biosensor as well as methods of using the biosensor to detect NAD.


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