The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 27, 2019

Filed:

Aug. 21, 2012
Applicants:

Atsushi Ideta, Kato-gun, JP;

Yoshito Aoyagi, Kato-gun, JP;

Inventors:

Atsushi Ideta, Kato-gun, JP;

Yoshito Aoyagi, Kato-gun, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/073 (2010.01); A01N 1/02 (2006.01); C12M 3/00 (2006.01); C12M 1/24 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0604 (2013.01); A01N 1/0221 (2013.01); C12M 21/06 (2013.01); C12M 23/08 (2013.01);
Abstract

Disclosed is a novel means which enables satisfactory preservation of embryos and fertilized eggs in the non-frozen state for a longer period than conventional means, which novel means also achieves high hatching ability and a high conception rate of the embryos after the preservation. The method for preserving a mammalian embryo(s) or fertilized egg(s) of the present invention comprises immersing a mammalian embryo(s) or fertilized egg(s) in a medium containing 20 to 80% (v/v) serum and 10 to 100 mM Good's buffer, and storing the embryo(s) or fertilized egg(s) at non-freezing low temperature. The preservative solution for a mammalian embryo(s) or fertilized egg(s) of the present invention essentially consists of a medium containing 20 to 80% (v/v) serum and 10 to 100 mM Good's buffer. The Good's buffer is preferably HEPES.


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