The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 27, 2019

Filed:

Aug. 07, 2015
Applicant:

The General Hospital Corporation, Boston, MA (US);

Inventors:

Mehmet Toner, Charlestown, MA (US);

Shannon Stott, Stoneham, MA (US);

Eduardo Reategui, Revere, MA (US);

Xiaocheng Jiang, Stoneham, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); C12N 5/09 (2010.01); G01N 33/543 (2006.01); G01N 33/574 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502761 (2013.01); C12N 5/0694 (2013.01); G01N 33/54366 (2013.01); G01N 33/57488 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0877 (2013.01); B01L 2400/086 (2013.01); C12N 2531/00 (2013.01);
Abstract

Methods and systems for isolating platelet-associated nucleated target cells, e.g., such as circulating epithelial cells, circulating tumor cells (CTCs), circulating endothelial cells (CECs), circulating stem cells (CSCs), neutrophils, and macrophages, from sample fluids, e.g., biological fluids, such as blood, bone marrow, plural effusions, and ascites fluid, are described. The methods include obtaining a cell capture chamber including a plurality of binding moieties bound to one or more walls of the chamber, wherein the binding moieties specifically bind to platelets; flowing the sample fluid through the cell capture chamber under conditions that allow the binding moieties to bind to any platelet-associated nucleated target cells in the sample to form complexes; and separating and collecting platelet-associated nucleated target cells from the complexes.


Find Patent Forward Citations

Loading…