The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 20, 2019

Filed:

Dec. 02, 2014
Applicant:

Biocartis N.v., Mechelen, BE;

Inventors:

Eva Servoli, Lausanne, CH;

Patrick Van Den Bogaard, Morges, CH;

Assignee:

Biocartis N.V., Mechelen, BE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); B01D 15/38 (2006.01); B01D 15/12 (2006.01); B01D 15/42 (2006.01); B01D 39/16 (2006.01); B01D 39/18 (2006.01); B01D 39/20 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1006 (2013.01); B01D 15/12 (2013.01); B01D 15/125 (2013.01); B01D 15/3885 (2013.01); B01D 15/424 (2013.01); B01D 39/16 (2013.01); B01D 39/18 (2013.01); B01D 39/2003 (2013.01); C12N 15/1013 (2013.01); C12N 15/1017 (2013.01); C12P 19/34 (2013.01); C12Q 1/6806 (2013.01);
Abstract

The present invention relates to a method for extracting circulating nucleic acids from a biological fluid. The method comprising the successive steps of providing the biological fluid supposed to contain the circulating nucleic acids. Then the biological fluid is contacted with a lysis solution comprising at least a chaotropic agent, a binding solution comprising at least a PEG derivative designed for cooperating with at least part of the circulating nucleic acids, wherein the binding solution is free of ethanol and isopropanol, and a solid support capable of capturing at least part of the circulating nucleic acid. Finally, the solid support is separated from the lysis solution, from the binding solution and from the biological fluid.


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