The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 20, 2019

Filed:

Oct. 15, 2018
Applicant:

The Trustees of the University of Pennsylvania, Philadelphia, PA (US);

Inventors:

James M. Wilson, Philadelphia, PA (US);

Anna Tretiakova, Woburn, MA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A61K 48/00 (2006.01); C12N 15/11 (2006.01); C12N 15/52 (2006.01); A61K 38/17 (2006.01); A61K 38/00 (2006.01); C07K 16/10 (2006.01); C12N 15/86 (2006.01); C12N 7/00 (2006.01); A61K 39/00 (2006.01);
U.S. Cl.
CPC ...
C07K 16/1018 (2013.01); A61K 48/0008 (2013.01); C12N 7/00 (2013.01); C12N 15/86 (2013.01); A61K 2039/507 (2013.01); C07K 2317/14 (2013.01); C07K 2317/31 (2013.01); C07K 2317/51 (2013.01); C07K 2317/515 (2013.01); C07K 2317/52 (2013.01); C07K 2317/622 (2013.01); C12N 2750/14121 (2013.01); C12N 2750/14143 (2013.01); C12N 2830/205 (2013.01); C12N 2830/30 (2013.01); C12N 2830/50 (2013.01); C12N 2840/203 (2013.01);
Abstract

A recombinant adeno-associated virus (AAV) having an AAV capsid and packaged therein a heterologous nucleic acid which expresses two functional antibody constructs in a cell is described. Also described are antibodies comprising a heavy chain and a light chain from a heterologous antibody. In one embodiment, the antibodies are co-expressed from a vector containing: a first expression cassette which encodes at least a first open reading frame (ORF) for a first immunoglobulin under the control of regulatory control sequences which direct expression thereof; and a second expression cassette which comprises a second ORF, a linker, and a third ORF under the control of regulatory control sequences which direct expression thereof, wherein the second and third ORF for a second and third immunoglobulin construct. The vector co-expressing these two antibody constructs is in one embodiment an AAV, in which the 5' and 3′ ITRs flank the expression cassettes and regulatory sequences.


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