The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 13, 2019

Filed:

May. 22, 2015
Applicants:

Advanced Liquid Logic, Inc., San Diego, CA (US);

Duke University, Durham, NC (US);

Inventors:

Ramakrishna Sista, Cary, NC (US);

Vamsee K. Pamula, Cary, NC (US);

Michael G. Pollack, Durham, NC (US);

Vijay Srinivasan, Cary, NC (US);

Allen E. Eckhardt, San Diego, CA (US);

Richard B. Fair, Durham, NC (US);

Assignees:

Advanced Liquid Logic, Inc., San Diego, CA (US);

Duke University, Durham, NC (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); G01N 27/447 (2006.01); G01N 33/543 (2006.01); C12M 1/12 (2006.01); G01N 35/10 (2006.01); G01N 35/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/54326 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502761 (2013.01); B01L 3/502784 (2013.01); C12M 25/01 (2013.01); C12M 25/16 (2013.01); G01N 27/44717 (2013.01); G01N 27/44786 (2013.01); G01N 33/54386 (2013.01); B01L 2200/025 (2013.01); B01L 2200/028 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0673 (2013.01); B01L 2200/141 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0427 (2013.01); G01N 35/0098 (2013.01); G01N 2035/1046 (2013.01);
Abstract

A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged for conducting droplet operations on a surface; (ii) a starting droplet comprising: (1) one or more magnetically responsive beads; (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone; (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet substantially lacking the magnetically responsive beads.


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