The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 13, 2019

Filed:

Mar. 05, 2018
Applicants:

Defeng Xing, Harbin, CN;

Najiaowa Yu, Harbin, CN;

Wei LI, Harbin, CN;

Zhen LI, Harbin, CN;

Yang Yang, Harbin, CN;

Inventors:

Defeng Xing, Harbin, CN;

Najiaowa Yu, Harbin, CN;

Wei Li, Harbin, CN;

Zhen Li, Harbin, CN;

Yang Yang, Harbin, CN;

Assignee:

Harbin Institute of Technology, Harbin, HeiLongJiang, CN;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 1/20 (2006.01); C12P 39/00 (2006.01); C12P 3/00 (2006.01); C12R 1/145 (2006.01); C07C 323/58 (2006.01); C07H 3/02 (2006.01); C07H 3/04 (2006.01); C12R 1/385 (2006.01);
U.S. Cl.
CPC ...
C12P 3/00 (2013.01); C07C 323/58 (2013.01); C07H 3/02 (2013.01); C07H 3/04 (2013.01); C12N 1/20 (2013.01); C12R 1/145 (2013.01); C12R 1/385 (2013.01);
Abstract

The present invention provides a method for fermentative hydrogen production under limited aerobic conditions by utilizing the respiratory interaction between a strictly anaerobic hydrogen producing bacterium,YUAN-3, and a facultative anaerobic bacterium,PAO1. The two bacteria are co-cultured to produce hydrogen gas in a culture medium without any anaerobic treatment. Sucrose, lactose or glucose are used as the carbon source for the co-culture which can promote the growth ofYUAN-3 and reduce substrate competition between two bacteria. L-cysteine is added to increase the hydrogen yield and the production rate. Using 15 g/L glucose and 5 mmol/L L-cysteine, the invented method achieved the hydrogen production yield of 1.11 mol-hydrogen/mol-glucose.


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