The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 13, 2019
Filed:
Sep. 01, 2015
BO Wang, Tempe, AZ (US);
Weiwen Zhang, Tianjin, CN;
Deirdre Meldrum, Phoenix, AZ (US);
ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY, Scottsdale, AZ (US);
Abstract
Methods of pre-methylation of foreign DNA to improve genetic transformation in cyanobacterium. Two Type II methyltransferase-encoding genes, i.e., M (sll0729) and C (slr0214), were cloned from the chromosome ofsp. PCC 6803 (hereafter6803) and expressed inthat harbors the integrative plasmid pBS-SPtK or pJU105. After pre-methylation in, the integrative plasmids were extracted and used for transformation of6803. The results showed that expression of slr0214 in the integrative-plasmid-harboringcells before DNA preparation resulted in orders of magnitude higher efficiency in the following integrative transformation of6803.