The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 06, 2019
Filed:
Jun. 17, 2014
Applicant:
Janssen Biotech, Inc., Horsham, PA (US);
Inventors:
Benjamin Fryer, Horsham, PA (US);
Daina Laniauskas, Horsham, PA (US);
Marcia Blackmoore, Horsham, PA (US);
Haiyun Wang, Horsham, PA (US);
Kostadinka Lilova, Spring House, PA (US);
Shelley Nelson, Titusville, NJ (US);
Elizabeth Rosocha, Raritan, NJ (US);
Assignee:
Janssen Biotech, Inc., Horsham, PA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/074 (2010.01); C12N 5/071 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0676 (2013.01); C12N 2500/25 (2013.01); C12N 2500/34 (2013.01); C12N 2501/117 (2013.01); C12N 2501/15 (2013.01); C12N 2501/16 (2013.01); C12N 2501/19 (2013.01); C12N 2501/385 (2013.01); C12N 2501/415 (2013.01); C12N 2501/727 (2013.01); C12N 2506/02 (2013.01);
Abstract
The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation. Specifically, the invention discloses methods of differentiating pluripotent cells into beta cell, cardiac cell and neuronal cell lineages using suspension clustering. The methods involve preparing the aggregated cell clusters followed by differentiation of these clusters.