The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 06, 2019

Filed:

Feb. 10, 2015
Applicant:

Technion Research & Development Foundation Limited, Haifa, IL;

Inventors:

Uri Sivan, Haifa, IL;

Elad Brod, Haifa, IL;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/32 (2006.01); B01L 3/00 (2006.01); C12M 1/00 (2006.01); C12M 3/00 (2006.01); C12M 3/06 (2006.01); C12M 1/36 (2006.01); G01N 33/569 (2006.01);
U.S. Cl.
CPC ...
C12M 23/12 (2013.01); B01L 3/5027 (2013.01); B01L 3/50273 (2013.01); B01L 3/50857 (2013.01); B01L 3/502753 (2013.01); C12M 21/08 (2013.01); C12M 23/16 (2013.01); C12M 29/00 (2013.01); C12M 41/48 (2013.01); C12M 47/02 (2013.01); G01N 33/56966 (2013.01); B01L 2300/069 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0829 (2013.01); B01L 2400/049 (2013.01); B01L 2400/0487 (2013.01);
Abstract

According to an aspect of some embodiments, an apparatus for isolation and cytometric analysis of cells from a liquid medium is provided. The apparatus comprises a cell reservoir, for receiving a liquid medium containing a suspension of cells. The apparatus further comprises a cell cage array between the cell reservoir and an enclosed reservoir, each cell cage comprising a large opening adjacent to the first cell reservoir, a cell cage, and one or more small opening adjacent to the enclosed reservoir. A connected liquid medium pump may move the suspended cells into cell cages by flowing the liquid medium from the cell reservoir to the enclosed reservoir. The apparatus further comprises a mechanical element which when actuated, pushes the cell cage array against a gel layer, forming a contiguous barrier to isolate each cell in a cell cage, and allowing cytometric analysis of isolated cells.


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