The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 06, 2019

Filed:

Apr. 14, 2016
Applicants:

Tokyo Women's Medical University, Tokyo, JP;

The Jikei University, Tokyo, JP;

Cellseed Inc., Tokyo, JP;

Inventors:

Takanori Hama, Tokyo, JP;

Hiromi Kojima, Tokyo, JP;

Hiroshi Moriyama, Tokyo, JP;

Masayuki Yamato, Tokyo, JP;

Teruo Okano, Tokyo, JP;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61F 2/18 (2006.01); C12N 5/0793 (2010.01); A61L 27/38 (2006.01); A61K 35/12 (2015.01);
U.S. Cl.
CPC ...
A61F 2/18 (2013.01); A61L 27/3804 (2013.01); A61L 27/3813 (2013.01); A61L 27/3839 (2013.01); A61L 27/3886 (2013.01); C12N 5/062 (2013.01); A61F 2002/183 (2013.01); A61F 2310/00365 (2013.01); A61K 35/12 (2013.01); A61L 2430/14 (2013.01); C12N 2533/30 (2013.01);
Abstract

The purpose of the present invention is to obtain an alternative to a substitute of the mucosa in the middle ear which is engrafted on the surface of the bone in the middle ear, hyperplasia of the granulation tissue and the bone and the development of the fibroblast cells in the middle ear are suppressed, and to obtain a middle ear mucosa-like cell sheet retaining cilia in the surface layer, comprising culturing nasal epithelium cells on a cell culture substrate coated with a polymer whose hydration force changes within a temperature range of 0 to 80° C., wherein the cells are cultured within a temperature range where the hydration force of the polymer is weak, and then changing the temperature to a temperature at which the hydration force is strong to recover the cultured cell sheet.


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