The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 30, 2019

Filed:

Sep. 11, 2017
Applicant:

Life Technologies Corporation, Carlsbad, CA (US);

Inventors:

Philip Martin, Carlsbad, CA (US);

Paul Nelson, Encinitas, CA (US);

David Bourdon, Carlsbad, CA (US);

Assignee:

LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/06 (2006.01); C12M 1/00 (2006.01); C07K 1/22 (2006.01); C07K 1/18 (2006.01); C07K 1/14 (2006.01); C07K 1/34 (2006.01);
U.S. Cl.
CPC ...
C12M 47/06 (2013.01); C07K 1/145 (2013.01); C07K 1/18 (2013.01); C07K 1/22 (2013.01); C07K 1/34 (2013.01); C12M 29/04 (2013.01); C12M 29/16 (2013.01);
Abstract

A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. A lysis solution is passed through the filter in a second flow direction opposite the first direction, thereby dislodging the plurality of cells captured on the filter, and resuspending the plurality of cells in the lysis solution on the first side of the filter. The lysis solution lyses the plurality of cells to produce a cellular lysate. The cellular lysate is passed through the filter in the first flow direction.


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