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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10362771 B1

PDFFull Text
Date of Patent:
Jul. 30, 2019

Filed:

Nov. 17, 2015

Method for knock-in of dna into target region of mammalian genome, and cell

Applicant:

Kyoto University, Kyoto-shi, Kyoto, JP;

Inventors:

Tomoji Mashimo, Kyoto, JP;

Kazuto Yoshimi, Kyoto, JP;

Takehito Kaneko, Kyoto, JP;

Assignee:

KYOTO UNIVERSITY, Kyoto, JP;

Attorney:

Leydig, Voit & Mayer, Ltd.

Primary Examiner:

Shin Lin Chen

Int. Cl.
CPC ...
C12N 15/63 (2006.01); C12N 15/00 (2006.01); C07H 21/04 (2006.01); C07K 14/00 (2006.01); A01K 67/027 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12N 15/90 (2006.01); C07K 14/315 (2006.01);
U.S. Cl.
CPC ...
A01K 67/0278 (2013.01); A01K 67/027 (2013.01); C12N 5/10 (2013.01); C12N 15/09 (2013.01); C12N 15/907 (2013.01); A01K 2207/15 (2013.01); A01K 2217/052 (2013.01); A01K 2217/072 (2013.01); A01K 2227/105 (2013.01); C07H 21/04 (2013.01); C07K 14/315 (2013.01); C12N 2800/10 (2013.01);
Abstract

This invention provides a method for knock-in of a donor DNA into the genome of a cell, comprising introducing at least one artificial nuclease system capable of cleaving target sequence(s) of the cell genome, the donor DNA, and two single-stranded oligonucleotides (ssODNs) into the cell, the artificial nuclease system cleaving the target sequence(s) on the cell genome, the two ssODNs each complementary to one of the ends generated by the target sequence cleavage in the cell genome and to one of the introduction ends of the donor DNA, the donor DNA being knocked-in at the cleavage site via the two ssODNs.

Find Patent Forward Citations

Mashimo, Tomoji. (2019). Method for knock-in of dna into target region of mammalian genome, and cell (U.S. Patent No. 10362771). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/10362771

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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