The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 23, 2019

Filed:

Jan. 14, 2016
Applicant:

Gpb Scientific, Llc, Richmond, VA (US);

Inventors:

Allyn Forsyth, San Diego, CA (US);

Helen Barnes, San Diego, CA (US);

Assignee:

GPB Scientific, LLC, Richmond, VA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/574 (2006.01); G01N 33/58 (2006.01); G01N 33/543 (2006.01); B01L 3/00 (2006.01); G01N 1/40 (2006.01);
U.S. Cl.
CPC ...
G01N 33/57492 (2013.01); B01L 3/502761 (2013.01); G01N 1/405 (2013.01); G01N 33/54326 (2013.01); G01N 33/574 (2013.01); G01N 33/585 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0861 (2013.01); B01L 2400/086 (2013.01); G01N 2333/705 (2013.01); Y10S 435/971 (2013.01); Y10S 435/973 (2013.01); Y10T 436/101666 (2015.01); Y10T 436/13 (2015.01); Y10T 436/25125 (2015.01); Y10T 436/25375 (2015.01);
Abstract

Method of enriching specific cells from cellular samples are disclosed, comprising contacting in solution a cellular sample with affinity-tagged ligands (ATLs) each comprising a first ligand linked to an affinity tag, wherein the ligand selectively binds a cellular marker of the rare cells and the affinity tag can be selectively captured by a capture moiety, wherein the affinity tags do not comprise a magnetic particle; and flowing the sample through a microfluidic device comprising the capture moiety to selectively retain ATL-bound cells. Methods for enriching circulating tumor cells, and devices for enriching specific cells from cellular samples are also disclosed.


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