The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 16, 2019

Filed:

Jul. 19, 2017
Applicant:

Institut Pasteur, Paris, FR;

Inventors:

Jean-Claude Manuguerra, Paris, FR;

Jessica Vanhomwegen, Paris, FR;

Philippe Despres, La Garenne-Colombes, FR;

Sylvie Paulous, Sarcelles, FR;

Assignee:

INSTITUT PASTEUR, Paris, FR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/53 (2006.01); C12Q 1/70 (2006.01); G01N 33/543 (2006.01); G01N 33/569 (2006.01); G01N 33/564 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
G01N 33/54306 (2013.01); G01N 33/54313 (2013.01); G01N 33/54353 (2013.01); G01N 33/564 (2013.01); G01N 33/56983 (2013.01); G01N 33/6845 (2013.01); G01N 2333/18 (2013.01); G01N 2333/185 (2013.01); G01N 2469/20 (2013.01); Y02A 50/51 (2018.01); Y02A 50/53 (2018.01); Y02A 50/56 (2018.01); Y02A 50/58 (2018.01); Y02A 50/60 (2018.01);
Abstract

The present invention provides an immunoassay leading to the rapid and simultaneous detection of antibodies to a wide range of infectious pathogens in biological fluids of infected patients. This immunoassay involves the covalent and oriented coupling of fusion proteins comprising an AGT enzyme and a viral antigen on an identifiable solid support (e.g. fluorescent microspheres). The thus obtained antigen-coupled microspheres show enhanced capture of specific antibodies as compared to antigen-coupled microspheres produced by standard amine coupling procedures.


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