The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 16, 2019
Filed:
Nov. 26, 2014
Applicant:
Mgi Tech Co., Ltd., Shenzhen, CN;
Inventors:
Chunyu Geng, Shenzhen, CN;
Ruoying Chen, Shenzhen, CN;
Yuan Jiang, Shenzhen, CN;
Xia Zhao, Shenzhen, CN;
Rongrong Guo, Shenzhen, CN;
Lingyu He, Shenzhen, CN;
Yaqiao Li, Shenzhen, CN;
Wenwei Zhang, Shenzhen, CN;
Hui Jiang, Shenzhen, CN;
Radoje Drmanac, Los Altos Hill, CA (US);
Assignee:
MGI TECH CO., LTD., Shenzhen, CN;
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6853 (2018.01); C12Q 1/6855 (2018.01); C12N 15/66 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1093 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6853 (2013.01); C12Q 1/6855 (2013.01); C12N 15/1089 (2013.01); C12N 15/66 (2013.01); C12Q 2525/191 (2013.01);
Abstract
Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.