The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 16, 2019

Filed:

Sep. 16, 2015
Applicants:

The Regents of the University of California, Oakland, CA (US);

Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA (US);

Inventors:

Kolin C. Hribar, La Jolla, CA (US);

Shaochen Chen, San Diego, CA (US);

Darren Finlay, La Jolla, CA (US);

Kristiina Vuori, La Jolla, CA (US);

Xuanyi Ma, La Jolla, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
B29C 41/02 (2006.01); C12N 5/00 (2006.01); B29C 64/124 (2017.01); B29C 64/135 (2017.01); B01L 3/00 (2006.01); C12M 1/26 (2006.01); B33Y 10/00 (2015.01); C12M 1/32 (2006.01); C12M 1/00 (2006.01); C12N 5/09 (2010.01); C12N 5/074 (2010.01); B29C 64/129 (2017.01);
U.S. Cl.
CPC ...
C12N 5/0062 (2013.01); B01L 3/5085 (2013.01); B29C 64/124 (2017.08); B29C 64/129 (2017.08); B29C 64/135 (2017.08); B33Y 10/00 (2014.12); C12M 23/12 (2013.01); C12M 23/22 (2013.01); C12M 33/00 (2013.01); C12N 5/0693 (2013.01); C12N 5/0696 (2013.01); B01L 2200/12 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0858 (2013.01); B01L 2300/0893 (2013.01); C12N 2513/00 (2013.01); C12N 2533/30 (2013.01);
Abstract

Using 3D printing, a microwell is formed by providing a plurality of masks, each mask representing a cross-section of a layer of the concave structure. Progressive movement of a projection plane exposes a pre-polymer solution to polymerizing radiation modulated by the masks to define the layers of the microwell, where each layer is exposed for a non-equal exposure period as determined by a non-linear factor. In a preferred embodiment, a first portion of the masks are base layer masks, which are exposed for a longer period than subsequent exposure periods. Shapes of the microwells, which may include circular, square, annular, or other geometric shapes, and their depths, are selected to promote aggregation behavior in the target cells, which may include tumor cells and stem cells.


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