The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 16, 2019

Filed:

Jun. 16, 2017
Applicant:

Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US);

Inventors:

Gang Chen, Yorktown Heights, NY (US);

Darya Burakov, Yonkers, NY (US);

Dipali Deshpande, White Plains, NY (US);

Assignee:

REGENERON PHARMACEUTICALS, INC., Tarrytown, NY (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K 16/22 (2006.01); C12N 15/85 (2006.01); C07K 14/47 (2006.01); C12N 15/79 (2006.01); C07K 16/00 (2006.01); C12N 9/24 (2006.01); C12N 15/09 (2006.01); C12N 15/63 (2006.01);
U.S. Cl.
CPC ...
C07K 16/22 (2013.01); C07K 14/47 (2013.01); C07K 14/4705 (2013.01); C07K 16/00 (2013.01); C12N 9/2488 (2013.01); C12N 15/09 (2013.01); C12N 15/79 (2013.01); C12N 15/85 (2013.01); C12Y 302/01 (2013.01); C07K 2317/14 (2013.01); C07K 2317/51 (2013.01); C07K 2317/515 (2013.01); C12N 15/63 (2013.01); C12N 2015/8518 (2013.01);
Abstract

The present invention relates to discovery of the ectopic expression of EDEM2 in a production cell to improve the yield of a useful multi-subunit protein. Thus, the present invention provides for production cell lines, such as the canonical mammalian biopharmaceutical production cell, the CHO cell, containing recombinant polynucleotides encoding EDEM2. Also disclosed is a production cell containing both an EDEM2-encoding polynucleotide as well an XBP1-encoding polynucleotide. Improved titers of antibodies produced by these cell lines are disclosed, as well as the improved cell densities attained by these cells in culture.


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