The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 09, 2019
Filed:
Apr. 08, 2016
Applicant:
Biolamina Ab, Sundbyberg, SE;
Inventors:
Agnete Kirkeby, Copenhagen, DK;
Malin Pernilla Parmar, Lund, SE;
Assignee:
BIOLAMINA AB, Sundbyberg, SE;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 35/30 (2015.01); A61K 35/545 (2015.01); C12N 5/0793 (2010.01); C12N 5/0797 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0623 (2013.01); A61K 35/30 (2013.01); A61K 35/545 (2013.01); C12N 5/0619 (2013.01); C12N 2500/38 (2013.01); C12N 2501/119 (2013.01); C12N 2501/13 (2013.01); C12N 2501/15 (2013.01); C12N 2501/41 (2013.01); C12N 2501/727 (2013.01); C12N 2501/999 (2013.01); C12N 2506/02 (2013.01); C12N 2533/52 (2013.01);
Abstract
The present disclosure relates to methods for producing dopaminergic cells and evaluating their functionality. When pluripotent human embryonic stem cells are cultured on plates coated with laminin-111, laminin-121, laminin-521, laminin-421, or laminin-511 in cell culture medium containing a GSK3 inhibitor and a TGF-β inhibitor as well as timely administered fibroblast growth factor, desired neural cells are produced at far higher rates. Useful cell culture kits for producing such dopaminergic cells are also described herein, as are methods of using such cells for stem cell therapy.