The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 02, 2019
Filed:
Nov. 01, 2018
Applicant:
Jiangnan University, Wuxi, CN;
Inventors:
Assignee:
JIANGNAN UNIVERSITY, Wuxi, CN;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/82 (2006.01); C12N 15/52 (2006.01); C12N 1/14 (2006.01); C12N 1/20 (2006.01);
U.S. Cl.
CPC ...
C12N 15/52 (2013.01); C12N 1/14 (2013.01); C12N 1/20 (2013.01); C12N 9/82 (2013.01); C12Y 305/01001 (2013.01);
Abstract
The present disclosure discloses an asparaginase mutant with efficient expression, activity and stability, belonging to the technical fields of gene engineering and enzyme engineering. The amino acid sequence of the asparaginase mutant is set forth in SEQ ID NO: 99, SEQ ID NO: 100 or SEQ ID NO: 101; meanwhile, compared with the wild type asparaginase mutant, the extracellular enzyme activity of the asparaginase fusion enzyme mutant is increased by up to 2.25 times, the stability is increased by up to 3.56 times, and the specific enzyme activity is increased by up to 1.34 times.