The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 02, 2019
Filed:
Nov. 16, 2018
Enzypep B.v., Geleen, NL;
Ana Toplak, Eindhoven, NL;
Timo Nuijens, Valkenburg, NL;
Peter Jan Leonard Mario Quaedflieg, Elsloo, NL;
ENZYPEP B.V., Geleen, NL;
Abstract
The invention relates to a subtilisin BPN' variant or homolog thereof, having the following mutations compared to subtilisin BPN′ represented by SEQUENCE ID NO: 2 or a homolog sequence thereof:—a deletion of the amino acids corresponding to positions 75-83;—a mutation at the amino acid position corresponding to S221, the mutation corresponding to S221C or S221 selenocysteine, preferably S221C;—at least one further mutation selected from the group consisting of amino acid positions corresponding to F189W, F189Y, S33D, S33T, N218D, N218T, N218E, N62D, N62S, N62W, and N62Y; preferably a mutation at the amino acid position corresponding to P225; wherein the amino acid positions are defined according to the sequence of subtilisin BPN′ represented by SEQUENCE ID NO: 2. The invention further relates to enzymatically synthesizing a peptide.