The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 25, 2019
Filed:
Apr. 20, 2016
Applicant:
Neogenomics Laboratories, Inc., Fort Myers, FL (US);
Inventor:
Maher Albitar, Valley Center, CA (US);
Assignee:
NEOGENOMICS LABORATORIES, INC., Fort Myers, FL (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12P 19/34 (2006.01); C12Q 1/6858 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6886 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6858 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6886 (2013.01); C12Q 2600/156 (2013.01);
Abstract
A method for detecting a low-occurrence mutation in isolated DNA adds a blocking probe to reagents during amplification of the isolated DNA. The blocking probe is an oligonucleotide complementary to wild-type DNA corresponding to the sample. The blocking probe spans a site of a suspected mutation within a region of interest in the isolated DNA. After amplification, fragments of the amplified DNA is sequenced using next generating sequencing and an output is generated to display the sequenced fragments. In some embodiments, the blocking probe is locked nucleic acid (LNA).