The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 25, 2019
Filed:
Nov. 18, 2016
Applicant:
Hoffmann-la Roche Inc., Little Falls, NJ (US);
Inventors:
Ulrich Goepfert, Penzberg, DE;
Benjamin Moritz, Munich, DE;
Assignee:
HOFFMANN-LA ROCHE INC., Little Falls, NJ (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/85 (2006.01); C12P 21/00 (2006.01); C12N 7/00 (2006.01); C07K 16/00 (2006.01);
U.S. Cl.
CPC ...
C12P 21/00 (2013.01); C07K 16/00 (2013.01); C12N 7/00 (2013.01); C12N 15/85 (2013.01); C07K 2317/14 (2013.01); C12N 2710/16122 (2013.01); C12N 2710/16143 (2013.01); C12N 2800/107 (2013.01); C12N 2830/46 (2013.01); C12N 2830/60 (2013.01);
Abstract
The current invention reports a promoter that has the nucleic acid sequence of SEQ ID NO: 02 or SEQ ID NO: 03 which is a human CMV major immediate-early (hCMV-MIE) promoter/enhancer with C to G point mutation at position −41 and/or −179 relative to the transcription start site. This new promoter is especially useful for the production of polypeptides at large scale as it shows reduced promoter silencing and improved polypeptide production.