The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 25, 2019

Filed:

Jun. 08, 2017
Applicant:

Sapporo Medical University, Chuo-ku, Sapporo-shi, Hokkaido, JP;

Inventor:

Osamu Honmou, Sapporo, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 35/12 (2015.01); A61K 35/16 (2015.01); C12N 5/0775 (2010.01); A61K 35/28 (2015.01);
U.S. Cl.
CPC ...
A61K 35/28 (2013.01); A61K 35/16 (2013.01); C12N 5/0663 (2013.01); A61K 35/12 (2013.01); A61K 2035/124 (2013.01); C12N 2500/84 (2013.01); C12N 2501/91 (2013.01);
Abstract

The present invention relates to a method for growing, rapidly and massively ex vivo, cells collected from a living subject to provide a safe and effective pharmaceutical preparation for biological tissue repair/regeneration. Specifically, the present invention relates to a method for growing cells in a sample collected from a living subject by culturing the cells in a medium containing allogeneic (including autogenic) serum. Preferably the allogeneic serum has been determined as being negative for a serum tumor marker and/or an infectious factors, and the amount of the anticoagulant (e.g., heparin, a heparin derivative, or a salt thereof) added to the collected sample is less than 5 U/mL with respect to the volume of the sample or the amount of the anticoagulant in the medium at the start of culture is less than 0.5 U/mL. The present invention further relates to use of the method.


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