The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 18, 2019
Filed:
Jun. 20, 2016
Konica Minolta Laboratory U.s.a., Inc., San Mateo, CA (US);
Noriaki Yamamoto, Tokyo, JP;
KONICA MINOLTA LABORATORY U.S.A., INC., San Mateo, CA (US);
Abstract
An improved method and related apparatus for detecting bacteria viability and drug effects using metabolic monitoring. A fluorescent material which is quenched by oxygen is co-localized with the target bacteria, and fluorescence signal is detected at the co-localized places. In some embodiments, the fluorescent material is a fluorescent nanoparticle mixed with the target bacteria in the sample, and co-localization is enhanced using centrifugation, electrophoresis, microflow path modified with antibodies, magnetic force, etc. In some other embodiments, the fluorescent material is a fluorescent film or 3-D matrix immobilized in the bacterial culture chamber, and bacteria in the sample is gathered into localized regions of the bacteria culture chamber where the fluorescent film or 3-D matrix is present by ways of centrifugation, electrophoresis or microflow path. Plasmonic nanoparticles with a metal core and plasmonic film with a metal film may be used as the fluorescent nanoparticles and fluorescent film.