The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 11, 2019
Filed:
Nov. 21, 2014
Mgi Tech Co., Ltd., Shenzhen, CN;
Yuan Jiang, Shenzhen, CN;
Kai Tian, Shenzhen, CN;
Xia Zhao, Shenzhen, CN;
Wenwei Zhang, Shenzhen, CN;
Huaiqian Xu, Shenzhen, CN;
Hui Jiang, Shenzhen, CN;
Radoje Drmanac, Los Altos Hills, CA (US);
Chunyu Geng, Shenzhen, CN;
MGI TECH CO., LTD., Shenzhen, CN;
Abstract
Provided is a method of constructing a sequencing library using an adaptor element in a bubble shape. The bubble-shaped adaptor is ligated to a DNA fragment respectively at the 3'-terminal and the 5′-terminal i.e., two bubble-shaped adaptors with same sequences are ligated in one step. The bubble-shaped adaptor-ligated product is then amplified with a primer complementary to the 3′-terminal of the long-chain nucleic acid of the bubble-shaped adaptor, so as to replace the non-paired sequence in the short-chain nucleic acid.