The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 11, 2019

Filed:

Jun. 26, 2014
Applicant:

Ecolab Usa Inc., Saint Paul, MN (US);

Inventors:

Anthony Wayne Erickson, Golden Valley, MN (US);

Elaine Patricia Black, St. Paul, MN (US);

Nathan Richard Ortmann, Buffalo, MN (US);

Rodney H. Banks, Aurora, IL (US);

Assignee:

Ecolab USA Inc., Saint Paul, MN (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/06 (2006.01); C12Q 1/24 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/06 (2013.01); C12Q 1/24 (2013.01);
Abstract

In situ optical analysis of bacterial endospores can be inhibited when the endospores are present within an optically active carrier medium. To help isolate the endospores from the carrier medium, in some examples, the carrier medium is passed through a hydrophobic material that captures the endospores via hydrophobic attraction. Subsequently, a germination fluid and lanthanide source, such as terbium, can be added to the bacterial endospores captured on the hydrophobic material to form a lanthanide-dipicolinic acid complex in the germination fluid. The germination fluid can then be optically analyzed by measuring the fluorometric response of the lanthanide-dipicolinic acid complex to determine a concentration of the bacterial endospores in the carrier medium.


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