The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 28, 2019
Filed:
Jan. 23, 2015
Centre National DE LA Recherche Scientifique (Cnrs), Paris, FR;
Universite Grenoble Alpes, St Martin d'Hères, FR;
Bernard Priem, Gieres, FR;
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS), Paris, FR;
UNIVERSITE GRENOBLE ALPES, Saint Martin d'Heres, FR;
Abstract
The present invention relates to a method for in vivo production of glycosaminoglycans (GAG), by metabolic engineering in a genetically modified cell. In a method according to the invention, said cell is genetically modified in order to express the genes coding for the enzymes that are suitable for synthesizing GAG from an exogenous precursor, preferably internalized by the cell. According to one specific feature, the present invention relates to a method for producing chondroitin or heparosan in a genetically modified bacterial cell, from an exogenous beta-galactoside precursor, preferably internalized by the cell. According to another specific feature, the present invention relates to the use of ancell comprising at least the genes glcA-T, kfoC, kfiD and wbpP for the production of chondroitin. According to yet another specific feature, the present invention relates to the use of ancell comprising at least the genes glcA-T, kfiA, kfiB, kfiC and kfiD for the production of heparosan. The present invention also relates to the use of the obtained glycosaminoglycans by implementing a method according to the invention in order to prepare a drug, a food composition or a cosmetic product.