The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 28, 2019
Filed:
Feb. 24, 2015
National Research and Development Agency National Agriculture and Food Research Organization, Tsukuba-shi, Ibaraki, JP;
Masaki Iwakami, Tsukuba, JP;
Seiichi Toki, Tsukuba, JP;
NATIONAL RESEARCH AND DEVELOPMENT AGENCY NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION, Tsukuba-shi, Ibaraki, JP;
Abstract
It has been found that a marker gene, which is inserted in the genomic DNA via homologous recombination, and to both ends of which nuclease recognition sites are added, can be removed from a plant cell by using a corresponding nuclease, and further that the nuclease recognition sites can also be removed without leaving any trace by matching sequences of at least 30 nucleotides adjacent to the recognition sites. Moreover, in a method for introducing a mutation into a target DNA on the genome of a plant cell via homologous recombination, it is made possible to: stably select a plant cell, in which the mutation is introduced, based on an expression of a marker gene; further, to remove an unnecessary sequence such as the marker gene from the selected cell; and to introduce only a required mutation into the target DNA.