The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 28, 2019

Filed:

Jan. 18, 2017
Applicant:

The Regents of the University of California, Oakland, CA (US);

Inventors:

Ellen May Sletten, Berkeley, CA (US);

Carolyn Ruth Bertozzi, Stanford, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07C 69/96 (2006.01); C07C 271/42 (2006.01); C07C 49/04 (2006.01); C07C 49/76 (2006.01); C07C 69/76 (2006.01); C07F 15/04 (2006.01); C12N 5/071 (2010.01); G01N 33/50 (2006.01); C07C 245/04 (2006.01); C07C 255/46 (2006.01); C07C 323/16 (2006.01); C07C 323/56 (2006.01); C07C 323/64 (2006.01); C07C 69/013 (2006.01); C07D 225/02 (2006.01); C07D 249/04 (2006.01); C07D 249/12 (2006.01); C07D 339/06 (2006.01); C07D 405/12 (2006.01); C07D 495/04 (2006.01);
U.S. Cl.
CPC ...
C07D 249/12 (2013.01); C07C 49/04 (2013.01); C07C 49/76 (2013.01); C07C 69/013 (2013.01); C07C 69/76 (2013.01); C07C 69/96 (2013.01); C07C 245/04 (2013.01); C07C 255/46 (2013.01); C07C 271/42 (2013.01); C07C 323/16 (2013.01); C07C 323/56 (2013.01); C07C 323/64 (2013.01); C07D 225/02 (2013.01); C07D 249/04 (2013.01); C07D 339/06 (2013.01); C07D 405/12 (2013.01); C07D 495/04 (2013.01); C07F 15/045 (2013.01); C12N 5/0602 (2013.01); G01N 33/5005 (2013.01); C07C 2601/14 (2017.05);
Abstract

The present disclosure features a strain-promoted [2+2+2] reaction that can be carried out under physiological conditions. In general, the reaction involves reacting a pi-electrophile with a low lying LUMO with a quadricyclane on a biomolecule, generating a covalently modified biomolecule. The selectivity of the reaction and its compatibility with aqueous environments provides for its application in vivo and in vitro. The reaction is compatible with modification of living cells. In certain embodiments, the pi-electrophile can comprise a molecule of interest that is desired for delivery to a quadricyclane-containing biomolecule via [2+2+2] reaction.


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